Lifeasible, a world leading biotechnology company specialized in agricultural science, now offers plant genetic modification by CRISPR/CAS9 to provided engineered plants with enhanced properties.
CRISPR/Cas9 gene editing is a technique for rapid editing of genomic DNA with very high efficiency and specificity, and its mechanism is the same as that used by prokaryotes to defend against viral or other foreign DNA invasion. Cas9 is an RNA-guided DNA endonuclease associated with CRISPR. SgRNAs (single-guideRNAs) are packaged with multiple crRNAs and tracrRNA that can bind Cas9 and direct this complex to complementary sequences in the target DNA. The HNH and RuvC domains of Cas9 then cleave DNA 3 bp upstream of the PAM (archetypal spacer adjacent motif) site (usually NGG), thus creating a DSB (DNA double-strand break). DSBs are repaired by NHEJ (non-homologous end joining) or HDR (homology-directed repair), allowing nucleotide insertion, deletion, substitution or site-specific mutation in the broken region. The simple design of sgRNAs strictly follows Watson-Crick base pairing, making CRISPR/Cas9 a very effective and promising gene editing tool.
Supported by years’ experience, Lifeasible has developed a transgenic plant platform based on CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein9) to provide engineered plants with enhanced properties such as high yields and nutrients, resistance to disease, pests, or drought tolerance.
Experts of the cell and molecular biology at Lifeasible are devoted to providing industry-leading custom sgRNA design and vector construction services to customers all over the world. Single and multiple genes knock-down, knock-out or knock-in, site-specific mutation even single nucleotide mutation according to alternative DNA repair pathways are available at Lifeasible. According to its official speaker, high throughput screening by the sgRNA library can also be performed.
“With fully-fledged transgenic technologies in many species such as Arabidopsis, rice, wheat, alfalfa, corn, cotton, sorghum, soybean, sugarbeet, cabbage, tobacco, eggplant, cucumber, potato, petunia, oilseed rape, we are equipped to serve the diverse requirements of our customers. We also have advanced CRISPR/Cpf1 system for more efficient and specific gene editing.” Commented one of the representative speakers from Lifeasible.
More information about the plant genetic modification by CRISPR/CAS9 provided by Lifeasible can be viewed here:.